With a rapidly changing climate, the effects of symbiotic bacteria and fungi on plant fitness and plant yield have become a new focus in both natural and agricultural populations. Yet many questions remain about even common and important members of the plant microbiome and the taxonomic scale at which they vary across plant compartments. Legumes are critical crops, cover crops, and members of natural communities, famously associating with nitrogen-fixing rhizobium but also forming symbiosis with other common taxa including bacteria in the genus Methylobacterium. Methylobacterium, known for their iconic red pigment and highly variable effects on plant growth and fitness, is associated with the phyllosphere and rhizosphere alike. GEMS trainee Allison Megow has developed a culture collection of more than 300 Methylobacterium strains collected from a long-term nitrogen experiment at Kellogg Biological Station, which has tested the effect of fertilization on plant communities for more than 30 years in order to investigate the implications of modern agricultural practices on Methylobacterium phylogenetics. She observed four distinct morphotypes evenly distributed across both nitrogen addition and control plots. To follow up on this observation, the 16S rRNA of a subset of these strains was sequenced with the aim of using these datasets to understand the eco-evolutionary implications of long-term nitrogen addition on communities of Methylobacterium associated with clover.