Insect microbiomes are remarkable subjects for studying host-microbe interactions, including host-parasite dynamics, as insects face strong selective pressure from obligate fungal parasites, such as behavior-altering fungi, but also retain obligate mutualists within endosymbiotic compartments for nutritional exchange. An extremely rare natural history event in North America will present the opportunity to study the periodical cicada, Magicicada spp. an elusive genus of cicada that emerges from soil in intervals of either thirteen or seventeen years. Illinois’s unique position, with both cicada broods emerging simultaneously for the first time since 1803, offers an unprecedented opportunity to study eco-evolutionary drivers shaping host-microbe interactions along the parasite-mutualism continuum, marking a historic event unseen in North America for two centuries. GEMS trainee Sierra Raglin will leverage the novelty of the dual periodical cicada emergence and collect specimens from both broods to characterize the spatiotemporal variation in periodical cicada microbiomes and study the environmental and host genetic drivers of nested genetic diversity within the periodical cicada.